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Registros recuperados: 13
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Analysis of diversity among six populations of Colombian mango (Mangifera indica L. cvar. Hilacha) using RAPDs markers Electron. J. Biotechnol.
Díaz-Matallana,Marcela; Schuler-García,Ingrid; Ruiz-García,Manuel; Hodson de Jaramillo,Elizabeth.
Currently in Colombia, there are only records of morph-agronomic characterizations of Mangifera indica cvar. Hilacha; molecular studies on this mango variety have not been carried out. The aim of this work was to identify the genetic diversity of six populations of mango Hilacha by RAPDs markers, as a fundamental base for breeding programs, conservation and selection of promissory materials for the fruit industry at the national level. From 60 primers evaluated in the populations, five primers were selected and were launched in the six populations. Polymorphic bands of RAPDs were transformed into binary matrices, which were then processed with NTSYS-PC, POPGENE and TFPGA softwares. The overall genetic diversity, H T = 0.468 ± 0.0016, is very similar to the...
Tipo: Journal article Palavras-chave: Genetic variability; Mango Hilacha; Organoleptic quality; RAPDs.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300001
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Caracterización de ADN de clones de papa e identificación de fitoplasmas asociados al síndrome de la punta morada Agrociencia
Alarcón-Rodríguez,Norma M.; Lozoya-Saldaña,Héctor; Valadez-Moctezuma,Ernestina.
En muchas áreas sembradas con papa en México se manifiesta el síndrome de la punta morada, que reduce el rendimiento y la calidad del tubérculo. Un factor principal de este síndrome es la presencia de fitoplasmas y el inadecuado manejo de su insecto vector. Una opción para controlar esta enfermedad es generar genotipos de papa resistentes y un diagnóstico oportuno para evitar su diseminación. Por ello los objetivos de este trabajo fueron detectar fitoplasmas asociados a la punta morada presentes en clones de papa asintomáticos, pero que estuvieron expuestos a la misma por cuatro ciclos, e identificar la relación hospedero-patógeno. Al final del ciclo de cultivo del 2005, fueron recolectadas hojas de 18 clones de papa sin síntomas, en Toluca, Estado de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Punta morada; Resistencia genética; RAPDs; RFLP.
Ano: 2009 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952009000400003
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CARACTERIZACIÓN molecular de AISLAMIENTOS DE Cladosporium fulvum Cooke PROVENIENTES DE TOMATE EN CONDICIONES DE CULTIVO PROTEGIDO Rev. Protección Veg.
Peteira,Belkis; Bernal Cabrera,A; Martínez,B; Ileana,Miranda.
El moho de la hoja, causado por el hongo Cladosporium fulvum Cooke, es una enfermedad muy común y destructiva en el cultivo del tomate. Aunque existen cultivares portadores de uno o más genes de resistencia, el hecho de que el hongo muta fácilmente trae como consecuencia un uso limitado de los mismos. De ahí que, el conocimiento de las poblaciones del patógeno contribuye a los esfuerzos realizados en el programa de mejoramiento y al desarrollo de estrategias que alarguen la vida útil de los materiales obtenidos. El objetivo de este trabajo fue la caracterización molecular de 37 aislados de C. fulvum provenientes de plantas de tomate de diferentes localidades. La caracterización incluyó la identificación de los aislamientos por la amplificación de la región...
Tipo: Journal article Palavras-chave: Cladosporium fulvum; Caracterización molecular; Genes Avr; Genes Ecp; RAPDs.
Ano: 2011 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522011000100002
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Diversidad genética de Phytophthora cinnamomi Rands de aguacate de Michoacán, México, por medio de RAPD Phyton
Ochoa-Fuentes,YM; Cerna,E; Landeros,J; Vazquez,O; Olalde,V; Flores,A.
Cuarenta y dos cepas de Phytophthora cinnamomi Rands de aguacate, de cinco municipios del estado de Michoacán, México, fueron examinados por polimorfismos de ADN amplificados al azar, con el objetivo de determinar la variabilidad genética. Dicha variabilidad se evaluó por reacción en cadena de la polimerasa con 21 cebadores de 10 bases, los cuales produjeron un total de 23 bandas. La distancia genética entre los aislados fue calculada por el método del promedio no ponderado y se obtuvo un dendrograma. El análisis mostró que las agrupaciones geográficas fueron fuertes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Persea americana; RAPDs; Tristeza del aguacatero.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000100005
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Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces Electron. J. Biotechnol.
Muthusamy,Saraladevi; Kanagarajan,Selvaraju; Ponnusamy,Shanmugasundaram.
Vigna umbellata (Thunb.) Ohwi and Ohashi commonly known as rice bean or climbing mountain bean is under-exploited tropical legume. Genetic variation between 10 landraces of rice bean was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. Among these markers, RAPD primers generated 987 amplification products of which 719 were polymorphic and ISSR markers produced 479 amplification products, out of which 296 were polymorphic. RAPD fingerprinting detected more polymorphic loci (70.30%) than the ISSR fingerprinting (61.79%). Mean PIC (polymorphism information content) for each of these marker systems (0.243 for RAPD and 0.203 for ISSR) suggested that both the marker systems were equally effective in...
Tipo: Journal article Palavras-chave: Genetic diversity; ISSRs; Landraces; Rice bean; RAPDs; Vigna umbellata.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300004
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Genetic diversity analysis of traditional and improved cultivars of Pakistani rice (Oryza sativa L.) using RAPD markers Electron. J. Biotechnol.
Rabbani,Malik Ashiq; Pervaiz,Zahida Hassan; Masood,Muhammad Shahid.
The molecular marker is a useful tool for assessing genetic variations and resolving cultivar identities. Information on genetic diversity and relationships among rice genotypes from Pakistan is currently very limited. The objective of this study was to evaluate the genetic polymorphisms and identities of 10 traditional, 28 improved and 2 Japanese cultivars of rice using the random amplified polymorphic DNA technique. Twenty-five decamer-primers could generate a total of 208 RAPD fragments, of which 186 or 89.4% were polymorphic. The number of amplification products produced by each primer varied from 4 to 16 with an average of 8.3 bands primer-1. The size of amplified fragments were ranged from 200 to 4000 bp. Pair-wise Nei and Li's similarity had...
Tipo: Journal article Palavras-chave: Cultivar discrimination; Genetic variability; Oryza sativa; Pakistani rice; RAPDs.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300006
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Genetic diversity of "Pimenta Longa" genotypes (Piper spp., Piperaceae) of the Embrapa Acre germplasm collection Genet. Mol. Biol.
Wadt,Lúcia Helena de Oliveira; Ehringhaus,Christiane; Kageyama,Paulo Yoshio.
The commonly known Pimenta longa is a commercially valuable natural resource found wild in Acre, Brazil. Specifically, three Piperaceae species with contested taxonomic status were studied, Piper hispidinervum, Piper aduncum, and Piper hispidum, to assesses the inter- and intra-specific genetic relationship of 49 Piper genotypes kept in the Pimenta longa germplasm collection at Embrapa Acre, using sixty six Random Amplified Polymorphic DNA (RAPD) markers. The DNA polymorphism level detected was high (96.97%), but the marker frequencies for each species showed polymorphism levels of 79.4% for Piper hispidinervum and 5.3% for P. aduncum. The genetic similarity clustering analysis resulted in three distinct groups corresponding to Piper hispidinervum, Piper...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Safrole; P. hispidinervum; P. aduncum; P. hispidum; RAPDs.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100013
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Morphological and genetic characteristics allow the identification of a collection of garlic cultivars in the North-Central region of Mexico Phyton
Azuara Hern ández,L; Silos Espino,H; Perales Segovia,C; Gómez Leyva,JF; Alpuche Solís,AG; Macias Valdez,LM.
The objective of this study was to compare the yield and genetic relationships between two Perla garlic selections, obtained by indi­vidual selection of cloves, and commercial varieties and cultivars adapted to diverse regions of Mexico (which did not have a selection process). Varieties with fewer cloves showed higher yields. Coreano and California produced 20 ton/ha (fresh weight) and Perla selections between 17-19 ton/ha (fresh weight). Six primers of the series OPB were tested for genetic characteriza-tion and OPB-17 was selected. With the amplified DNA fragments, a binary matrix was generated; afterwards, a dissimilarity matrix and dendrogram were developed. High genetic diversity was found among all varieties, which were separated into two groups...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RAPDs; Allium sativum; Selection; Yield.
Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572008000100007
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Morphological and genetic differentiation among Chilean populations of Bufo spinulosus (Anura: Bufonidae) RChHN
Méndez,Marco A.; Soto,Eduardo R.; Correa,Claudio; Veloso,Alberto; Vergara,Eliseo; Sallaberry,Michel; Iturra,Patricia.
Bufo spinulosus has a wide and fragmented range distribution in Chile (18° to 33° S) along altitudinal and latitudinal gradients. Genetic variation was estimated using RAPD (Random Amplified Polymorphic DNA) markers in 10 populations from northern and central Chile. Morphometric and genetic information was analyzed as a function of geographical origin. The correlation between genetic and morphometric differentiation was analyzed by the Mantel test. An increase in body size as a function of latitude was observed. Specimens from El Tatio had the smallest body size and the greatest morphometric divergence. The AMOVA applied to genetic data indicated that 57.85 % of the variance is explained by interregional differences and that 30.12 % of the...
Tipo: Journal article Palavras-chave: Amphibians; Geographic variation; Morphometry; RAPDs; AMOVA.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2004000300014
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Phylogenetic relationships among species of the subsection Dendrophlomis Bentham Electron. J. Biotechnol.
Yuzbasioglu,Ertugrul; Dadandi,Mehmet Yasar.
This study used randomly amplified polymorphic DNA markers to determine genetic relationships among species of the subsection Dendrophlomis. Twenty accessions of the eleven Phlomis taxa were evaluated to determine genetic variability using fourteen ten mer primers selected from a 125 random oligonucleotide set. These 14 selected primers generated 85 RAPD bands that ranged in size from 200 to 1200 base pairs. Of the total bands, 88% (75) were polymorphic among the samples. Genetic distances among accessions were computed to produce a dendrogram based on UPGMA. Genetic distances ranged from 0.133 (between P. amanica and P. monocephala) to 0.494 (between P. chimerae and P. lunariifolia). The UPGMA tree based on distances has two major groups. The first...
Tipo: Journal article Palavras-chave: Genetic diversity; Genetic relationships; Genetic variation; Phlomis species; RAPDs.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400012
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Small-scale morphological and genetic differentiation in the Mediterranean killifish Aphanius fasciatus (Cyprinodontidae) from a coastal brackish-water pond and an adjacent pool in northern Sardinia ArchiMer
Maltagliati, F; Domenici, P; Fosch, Cf; Cossu, P; Casu, M; Castelli, A.
Two samples of Aphanius fasciatus collected in the Pilo pond (northern Sardinia, Italy) and in an adjacent pool of small surface area were analysed morphologically (235 individuals) and genetically (a subsample of 58 individuals). The aims of the present study were (i) to test the hypothesis that different predation pressures may be associated with morphological and/or genetic differences between samples from each habitat and (ii) to assess the level of divergence between the two populations. Morphological analysis was based on the relative size of fins because it has been shown to be associated with predation pressure. The relative caudal fin area (caudal fin area/total body surface) was smaller in specimens from the pool, in both males and females,...
Tipo: Text Palavras-chave: Morphologie; Taille relative des nageoires; Variation génétique; Divergence de populations; Eaux saumâtres; Morphology; Relative fin size; Genetic variation; RAPDs; Population divergence; Brackish-water habitats.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00322/43291/43026.pdf
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Variabilidad genética en 22 variedades híbridas de caña de azúcar (Saccharum spp. Híbrido) Phyton
Alejandre Rosas,JA; Galindo Tovar,ME; Lee Espinosa,HE; Alvarado Gómez,OG.
El desarrollo de nuevas variedades en caña de azúcar permite aumentar la producción de sacarosa, y enfrentar grandes desafíos como nuevas plagas y la pérdida de fertilidad de los suelos. El propósito del presente estudio fue aportar herramientas para la selección de progenitores útiles en los programas de cruzamiento. Para ello, se utilizaron marcadores moleculares RAPDs; se probaron 14 iniciadores y se seleccionaron los cuatro que generaron información suficiente para realizar el análisis genético de 22 variedades híbridas. Éstas fueron usadas como progenitores en los programas de hibridación en la Estación de Hibridación, Cámara Nacional de la Industria Azucarera y Alcoholera, Tapachula, Chiapas. En el análisis de distancia genética se obtuvieron tres...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Marcadores moleculares; RAPDs; Caña de azúcar; Selección; Mejoramiento genético.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000100013
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Variación morfométrica y genética de Diaphorina citri (Hemiptera: Liviidae) en rutáceas de Veracruz, México. Colegio de Postgraduados
Cuellar Sánchez, Valeria Isabel.
Diaphorina citri es el vector más importante del Huanglongbing, la enfermedad más destructiva de los cítricos en el mundo. El objetivo de este estudio fue detectar la presencia de variantes morfométricas y genéticas, a través del análisis de secuencias de nucleótidos mediante RAPDs y el gen mitocondrial mtCOI para determinar si existe correspondencia entre variantes de individuos de D. citri Kuwayama (Hemiptera: Liviidae), colectados en rutáceas de Veracruz, México. Se encontraron variaciones morfométricas en el ancho, largo y venación de las alas y de los procesos genales de los especímenes estudiados. Los análisis genéticos por medio de RAPDs y secuencias obtenidas con el gen mtCOI confirman la existencia de cuatro haplotipos (H1-H4) de D. citri...
Palavras-chave: Psílido asiático de los cítricos; Morfometría; RAPDs; COI; PCR; Asiatic citrus psyllid; Morphometrics; Entomología y Acarología; Maestría.
Ano: 2014 URL: http://hdl.handle.net/10521/2346
Registros recuperados: 13
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